| Saddleback Caterpillar Moth | Acharia | stimulea | | 1 | Limacodidae | Lepidoptera | Insecta | Animalia |
| Mountain Earthsnake | Virginia | valeriae pulchra | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| Timber Rattlesnake | Crotalus | horridus | | 1 | Crotalidae | Squamata | Reptilia | Animalia |
| Honeydew Eater | Scorias | spongiosa | | 1 | Capnodiaceae | Capnodiales | Dothideomycetes | Fungi |
| American Water Plantain | Alisma | subcordatum | | 1 | Alismataceae | Alismatales | Magnoliopsida | Plantae |
| Harbinger of Spring | Erigenia | bulbosa | | 1 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| Blue Mistflower | Conoclinium | coelestinum | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| White Wood Aster | Eurybia | divaricata | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Rattlesnake Weed | Hieracium | venosum | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Tall Rattlesnake Root | Nabalus | altissimus | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| White Goldenrod | Solidago | bicolor | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Great Blue Lobelia | Lobelia | siphilitica | | 1 | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| Virginia Springbeauty | Claytonia | virginica | | 1 | Montiaceae | Caryophyllales | Magnoliopsida | Plantae |
| American Pokeweed | Phytolacca | americana | | 1 | Phytolaccaceae | Caryophyllales | Magnoliopsida | Plantae |
| Flowering Dogwood | Cornus | florida | | 2 | Cornaceae | Cornales | Magnoliopsida | Plantae |
| Spotted Wintergreen | Chimaphila | maculata | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Eastern Teaberry | Gaultheria | procumbens | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Pinesap | Monotropa | hypopitys | | 2 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Indian Pipe | Monotropa | uniflora | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Large Cranberry | Vaccinium | macrocarpon | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Deerberry | Vaccinium | stamineum | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| American Hazelnut | Corylus | americana | | 1 | Betulaceae | Fagales | Magnoliopsida | Plantae |
| Common Milkweed | Asclepias | syriaca | | 1 | Apocynaceae | Gentianales | Magnoliopsida | Plantae |
| Whorled Milkweed | Asclepias | verticillata | | 1 | Apocynaceae | Gentianales | Magnoliopsida | Plantae |
| Green Milkweed | Asclepias | viridiflora | | 1 | Apocynaceae | Gentianales | Magnoliopsida | Plantae |
| Virginia Pennywort | Obolaria | virginica | | 1 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Yellow Jewelweed | Impatiens | pallida | | 1 | Balsaminaceae | Geraniales | Magnoliopsida | Plantae |
| Virginia Bluebells | Mertensia | virginica | | 2 | Boraginaceae | Lamiales | Magnoliopsida | Plantae |
| Wild Bergamot | Monarda | fistulosa | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Hairy Skullcap | Scutellaria | elliptica var. elliptica | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Beechdrops | Epifagus | virginiana | | 2 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Allegheny Monkeyflower | Mimulus | ringens var. ringens | | 1 | Phrymaceae | Lamiales | Magnoliopsida | Plantae |
| Yellow Trout Lily | Erythronium | americanum ssp. americanum | | 1 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Flypoison | Amianthium | muscitoxicum | | 1 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Pawpaw | Asimina | triloba | | 1 | Annonaceae | Magnoliales | Magnoliopsida | Plantae |
| Puttyroot | Aplectrum | hyemale | | 1 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Bloodroot | Sanguinaria | canadensis | | 1 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Tawny Cottongrass | Eriophorum | virginicum | | 1 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Twinleaf | Jeffersonia | diphylla | | 1 | Berberidaceae | Ranunculales | Magnoliopsida | Plantae |
| White Baneberry | Actaea | pachypoda | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Black Baneberry | Actaea | racemosa | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Round-lobed Hepatica | Anemone | americana | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Early Meadow-rue | Thalictrum | dioicum | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| American Alumroot | Heuchera | americana | | 2 | Saxifragaceae | Rosales | Magnoliopsida | Plantae |
| Oneseed Cucumber | Sicyos | angulatus | | 1 | Cucurbitaceae | Violales | Magnoliopsida | Plantae |
| Mountain Spleenwort | Asplenium | montanum | | 2 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Ebony Spleenwort | Asplenium | platyneuron | | 1 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Wallrue | Asplenium | ruta-muraria | | 1 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Maidenhair Spleenwort | Asplenium | trichomanes ssp. trichomanes | | 1 | Aspleniaceae | Polypodiales | Polypodiopsida | Plantae |
| Common Polypody | Polypodium | virginianum | | 1 | Polypodiaceae | Polypodiales | Polypodiopsida | Plantae |
| Purple Cliffbrake | Pellaea | atropurpurea | | 1 | Pteridaceae | Polypodiales | Polypodiopsida | Plantae |