| Fowler's Toad | Anaxyrus | fowleri | | 1 | Bufonidae | Anura | Amphibia | Animalia |
| Green Frog | Lithobates | clamitans | | 1 | Ranidae | Anura | Amphibia | Animalia |
| Yellow Garden Spider | Argiope | aurantia | | 1 | Araneidae | Araneae | Arachnida | Animalia |
| Golden Eagle | Aquila | chrysaetos | | 1 | Accipitridae | Accipitriformes | Aves | Animalia |
| Bufflehead | Bucephala | albeola | | 1 | Anatidae | Anseriformes | Aves | Animalia |
| Ruddy Duck | Oxyura | jamaicensis | | 1 | Anatidae | Anseriformes | Aves | Animalia |
| Common Loon | Gavia | immer | | 1 | Gaviidae | Gaviiformes | Aves | Animalia |
| Kentucky Warbler | Geothlypis | formosa | | 1 | Parulidae | Passeriformes | Aves | Animalia |
| Brown-headed Nuthatch | Sitta | pusilla | | 1 | Sittidae | Passeriformes | Aves | Animalia |
| Eastern Kingbird | Tyrannus | tyrannus | | 1 | Tyrannidae | Passeriformes | Aves | Animalia |
| Great Blue Heron | Ardea | herodias | | 1 | Ardeidae | Pelecaniformes | Aves | Animalia |
| American White Pelican | Pelecanus | erythrorhynchos | | 1 | Pelecanidae | Pelecaniformes | Aves | Animalia |
| Margined Leatherwing | Chauliognathus | marginatus | | 1 | Cantharidae | Coleoptera | Insecta | Animalia |
| Dogbane Beetle | Chrysochus | auratus | | 1 | Chrysomelidae | Coleoptera | Insecta | Animalia |
| No Common Name | Acilius | mediatus | | 1 | Dytiscidae | Coleoptera | Insecta | Animalia |
| Sand Hammertail | Efferia | albibarbis | | 1 | Asilidae | Diptera | Insecta | Animalia |
| Red-footed Cannibalfly | Promachus | rufipes | | 3 | Asilidae | Diptera | Insecta | Animalia |
| Tiger Bee Fly | Xenox | tigrinus | | 1 | Bombyliidae | Diptera | Insecta | Animalia |
| Two-spotted Bumble Bee | Bombus | bimaculatus | | 1 | Apidae | Hymenoptera | Insecta | Animalia |
| Sweat Bee | Augochlorella | sp. | | 1 | Halictidae | Hymenoptera | Insecta | Animalia |
| Giant Resin Bee | Megachile | sculpturalis | | 1 | Megachilidae | Hymenoptera | Insecta | Animalia |
| No Common Name | Eremnophila | aureonotata | | 2 | Sphecidae | Hymenoptera | Insecta | Animalia |
| Small Phigalia Moth | Phigalia | strigataria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Sachem | Atalopedes | huron | | 1 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Silver-spotted Skipper | Epargyreus | clarus | | 1 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Horace's Duskywing | Erynnis | horatius | | 2 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Zabulon Skipper | Lon | zabulon | | 1 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Gray Hairstreak | Strymon | melinus | | 1 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Drexel's Datana Moth | Datana | drexelii | | 1 | Notodontidae | Lepidoptera | Insecta | Animalia |
| Monarch | Danaus | plexippus | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Variegated Fritillary | Euptoieta | claudia | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Appalachian Brown | Lethe | appalachia | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Pearl Crescent | Phyciodes | tharos | | 2 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Red Admiral | Vanessa | atalanta | | 1 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Zebra Swallowtail | Eurytides | marcellus | | 2 | Papilionidae | Lepidoptera | Insecta | Animalia |
| Eastern Tiger Swallowtail | Pterourus | glaucus | | 1 | Papilionidae | Lepidoptera | Insecta | Animalia |
| Sleepy Orange | Abaeis | nicippe | | 1 | Pieridae | Lepidoptera | Insecta | Animalia |
| Luna Moth | Actias | luna | | 1 | Saturniidae | Lepidoptera | Insecta | Animalia |
| Pandorus Sphinx | Eumorpha | pandorus | | 1 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Hummingbird Clearwing | Hemaris | thysbe | | 1 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Common Whitetail | Plathemis | lydia | | 1 | Libellulidae | Odonata | Insecta | Animalia |
| Wrinkled Grasshopper | Hippiscus | ocelote | | 1 | Acrididae | Orthoptera | Insecta | Animalia |
| American Shieldback | Atlanticus | americanus | | 1 | Tettigoniidae | Orthoptera | Insecta | Animalia |
| Delmarva Fox Squirrel | Sciurus | niger cinereus | | 1 | Sciuridae | Rodentia | Mammalia | Animalia |
| Eastern Copperhead | Agkistrodon | contortrix | | 1 | Crotalidae | Squamata | Reptilia | Animalia |
| Common Five-lined Skink | Plestiodon | fasciatus | | 1 | Scincidae | Squamata | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 2 | Emydidae | Testudines | Reptilia | Animalia |
| Cedar Apple Rust | Gymnosporangium | juniperi-virginianae | | 1 | Gymnosporangiaceae | Pucciniales | Pucciniomycetes | Fungi |
| Cardinal Flower | Lobelia | cardinalis | | 1 | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| American Holly | Ilex | opaca | | 1 | Aquifoliaceae | Celastrales | Magnoliopsida | Plantae |
| Spotted Wintergreen | Chimaphila | maculata | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Panicledleaf Tick-trefoil | Desmodium | paniculatum var. paniculatum | | 1 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Rosepink | Sabatia | angularis | | 1 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Watershield | Brasenia | schreberi | | 1 | Cabombaceae | Nymphaeales | Magnoliopsida | Plantae |
| American White Water-lily | Nymphaea | odorata ssp. odorata | | 2 | Nymphaeaceae | Nymphaeales | Magnoliopsida | Plantae |